| Bisons Track Club | Blue Devils AC |
| Brampton Racers TC | Brampton Racers Track & Field Club |
| Brantford TFC | Burlington TFC |
| CK Running Club | Crown Athletics |
| Durham Dragons | Eclipse Track & Field Club |
| Emmanuel Christian High School | Empire track |
| Etobicoke Track | Flying Angels |
| Flying Angels TF Academy | Georgian Bay Coyotes |
| Hamilton Olympic Club | Helen Detwiler - HWDSB |
| Invictus Athletics | Kitchener Waterloo TFA |
| Laurel Creek TFC | Maximus Performance |
| Mississauga Olympians | Mississauga Olympians MTA |
| Newmarket Huskies TC | No team |
| Oakville Renegades Track Club | Oakville Track & Field Club Athletiques International |
| PACK Running Club | Project Go |
| RoadRunners Track & Field | Simple Fitness Track Club |
| SOUTH GREY ATHLETICS | South Simcoe Dufferin TFC |
| Speed Dee AC | STAC Hamilton |
| Stratford Sabrecats Track Club | The Gazelles Track and Field Club |
| Thorold Elite TC | Thorold Elite Track Club |
| Toronto West Athletics | unassigned |
| Unattached Ontario | United Tamil SC |
| University of Toronto TC | Woodstock Legion AC |
| Woodstock Legion Athletic Club | York Region Runners |
| York Region Runners Club |