
| 310 Running | Athletics Niagara |
| Blue Devils Athletics Club | Boardwalk RC |
| BOOST Athletics Club | Brampton Elite TC |
| Brampton Racers TC | Brantford TFC |
| Bullets Track | Burlington Track & Field Club |
| Durham Dragons | Durham Legion Athletics |
| Etobicoke Track | Excel Athletics |
| Flying Angels Academy | Flying Angels Academy |
| Georgian Bay Coyotes | Glen Manor Track Club |
| I Be Fast Track Club | Independent |
| Invictus Athletics | Kitchener Waterloo TFA |
| London Legion TF Alliance | Maximus Performance |
| Milton Leap Squad | Milton Spikes |
| Milton Spikes Track and Field Club | Mississauga TFC |
| Monte Cristo Track Club | Newmarket Huskies TC |
| Peel Panthers | Peel Panthers SC |
| Project Athletics | South Simcoe Dufferin TFC |
| Speed City TC | Super Dupers |
| The Gazelles Track and Field Club | Thorold Elite TC |
| Toronto Tornadoes | United Tamil SC |
| Variety Village Athletic Club | York Region Runners Club |