| 310 Running | Airblastoff Lions ABOL |
| Athletics Niagara | Blue Devils AC |
| Blue Devils Athletics Club | Boardwalk RC |
| BOOST Athletics Club | Brampton Elite TC |
| Brampton Racers TC | Brantford TFC |
| Brockville Legion TC | Bullets Track |
| Burlington TFC | Centro Scuola TFC |
| Durham Dragons | Durham Legion Athletics |
| Eclipse Track & Field Club | Excel Athletics |
| Flying Angels TF Academy | Georgian Bay Coyotes |
| Guelph Minor TFC | Hamilton Olympic Club |
| I Be Fast TC | Invictus Athletics |
| Kitchener Waterloo TFA | London Legion TF Alliance |
| Maximus Performance | Milton Leap Squad |
| Milton Spikes | Milton Spikes Track and Field Club |
| Mississauga Olympians | Mississauga TFC |
| Monte Cristo Track Club | Newmarket Huskies TC |
| North Simcoe | Oakville Renegades Track Club |
| Project Athletics | Quinte Legion Lynx Track and Field Club |
| Rivers Speed Stars | Saugeen TFC |
| Shahph Puma SC | SOUTH GREY ATHLETICS |
| South Simcoe Dufferin TFC | Speed City TC |
| Stratford Sabrecats Track Club | Thorold Elite TC |
| Time Bandits | Timmins Porcupine TFC |
| Toronto Tornadoes | Toronto West Athletics |
| United Tamil SC | York Region Runners |
| York University TFC |