| Border City AC | Burlington Track & Field Club |
| Golden Eagles Track Club | Hamilton Olympic Club |
| Laurel Creek T.F.C | London Western T.F.C |
| Monte Cristo Track Club | Niagara Olympic Club |
| Oakville Track & Field Club Athletiques International | Royal City Athletics Club |
| Stratford Sabrecats Track Club | Sundown Athletics Club |
| Thorold Elite Track Club | University of Windsor Athletics Club |
| UNON - Unattached Ontario | Variety Village Athletic Club |
| Windsor Legion T.F.C | Woodstock Legion Athletic Club |