| Athletiques International | Boardwalk RC |
| Brampton Racers TC | Brantford TFC |
| Burlington TFC | Centro Scuola T.F.C. |
| CK Running Club | Cornerstone |
| Durham Dragons | Etobicoke Track |
| Flying Angels TF Academy | Golden Eagle Track Club |
| Grandpa | Hamilton Olympic Club |
| Invictus Athletics | Kitchener Waterloo T.F.A. |
| Maximus Performance | Milton Spikes Track and Field Club |
| Mississauga Olympians | Monte Cristo Track Club |
| Newmarket Huskies TC | Newmarket Huskies Track Club |
| Oakville Renegades Track Club | Quest Sports Track and Field Club |
| South Simcoe Dufferin TFC | The Gazelles Track and Field Club |
| Thorold Elite TC | Toronto West Athletics |
| Unattached Ontario | United Tamil SC |
| York Region Runners | York Region Runners Club |