| 2016 Alberta Summer Games | 310 Running |
| Agape Tornadoes | Athletics Niagara |
| Blue Devils Athletics Club | Bolton Pole Vault |
| Burlington Track & Field Club | C.A.N.I. Athletics |
| Central Ontario Rogues | Central Toronto Athletic Club |
| Chinguacousy Secondary School | Durham Dragons Athletics |
| Flying Angels Academy | Guelph Gryphons |
| Laurel Creek T.F.C | Monte Cristo Track Club |
| Okanagan Athletics Club | Phoenix Athletics Assoc. of Ontario |
| Physi-Kult | Speed River Track & Field Club |
| Supreme Athletics | The Gazelles |
| Toronto Metro Bold | Toronto Olympic Track & Field Club |
| Toronto Varsity Blues | Toronto West Athletics |
| University of Toronto - Mississauga | University of Toronto TC |
| Uxbridge (Unattached) | Waterloo Warriors |
| York Lions | York University Track & Field Club |